GROWTH CULTURE MEDIA SERIES 2: Pathological Evaluation of Ofor (Detarium microcarpum) Dextrose Broth (SDB) for the Growth of Fungal Cultures.
Cooked Ofor fruit (Detarium microcarpum) were plated using standard blotter method, to evaluate its potential as a medium for fungal growth. Four different fungi such as; Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum, Fusarium oxysporium, and Rhizopus stolonifer were obtained. Healthy fruits were used to prepare natural growth medium which pure cultures of the different organisms were transferred into. From the results obtained, the medium did not support so much growth and when evaluated, it was discovered that the poor growth was due to the presence of some antinutritional factors such as oxalate, phytate, saponin and tannin. These antinutritional factors affect the availability of nutrients required by the body of the fungi. It interfered with metabolic process so that growth and bioavailability of nutrients are negatively influenced.
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