From Biochemical Assays in Cells to Applications in Ranges: An Antidotal Treatment of Intoxication by Pussley (Portulaca oleracea)

Bilal Ghareeb


The assessment of the cytotoxicity of pussley (Portulaca oleracea L.) enrolls in the first series of studies of ex vivo evaluation of toxicity of range plants in Palestine. In parallel, this study looks for an antidotal treatment in Milk Thistle (Silybum marianum) which is available in the Palestinian environment. This study has an economical, ecological, agricultural and political importance through preserving livestock, ranges and land…

Starting at 500 µg of Portulaca oleracea extract/ml of HepG2 cell culture medium, a clear toxicity for HepG2 cell line as expressed by MTT viability test is demonstrated as directly proportional to the plant dose. Silybum marianum shows a similar profile even though with a viability profile weaker than that of Portulaca oleracea. This is unexpected for a plant (Silybum marianum) used classically as hepatic regenerative and antidote agent, recall that Portulaca oleracea is an edible plant.

However, Silybum marianum is demonstrated to be efficient (at 1.5 mg of plant extract/ml of HepG2 cell line culture medium) as an antidote against the toxicity of Portulaca oleracea. This opens horizons for using Silybum marianum as an antidote in case of intoxication of livestock and also humans.


Plants toxicity; livestock; pussley (Portulaca oleracea L.) and antidote effects of milk thistle (Silybum marianum).

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